Genomic Insight and Optimization of Astaxanthin Production from a New Rhodotorula sp. CP72-2

Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-2, isolated from Calotropis gigantea, showed potential astaxanthin production. In this study, strain CP72-2 was identified as putative new species the genus based on 26S rRNA gene sequence (98% identity). It first microbial source for producing astaxanthin. Strain screened its production and quantified by High-Performance Liquid Chromatography (HPLC), Chromatography-Mass Spectrometry (LC-MS), UV-Vis spectrophotometer. After screening of production, carbon sources, pH, temperature, incubation period were evaluated their effect CP72-2. Among several experimental factors, most efficient conditions glucose (50 g/L), pH 4.5, 25 °C, three days cultivation. The assembly genome has total length 21,358,924 bp GC content 64.90%. candidate biosynthesis-associated genes (i.e., CrtE, CrtYB, CrtI, CrtS, CrtR, CrtW, CrtO, CrtZ) found. This research presents report optimization analysis, focusing biotechnological producer.